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mercredi 18 juin 2014

The Importance Of Cancer ELISA Kits

By Lila Bryant


The enzyme linked immunosorbent assay or ELISA has been used as a tool in detecting the presence of a substance that is called an antigen in a wet or liquid sample. The cancer ELISA kits are used as a tool in recognizing any elements of any chemicals and responses from the immune system for any contagious microbes. The tests include an enzyme that is a result of a biochemical reaction. It includes an antigen molecules in the body.

Cancer Antigen 125 is a quickly quantify antigen concentrations in human serum. It has three features, it is easy, efficient and rapid. It is easy because it has features fixed in one system and no required additional equipment needed. It is efficient because the test can reach for 96 samples at once. It can give results in just less than 4 hours. The technology can allow you to screen any multiple serum samples for several antigens associated with cancers in a present and elevated levels.

Another is the breast cancer susceptibility protein 1 or the BRCA1. This is a high sensitive kit to specify and detect anyone who has breast tumor. This kit is identified by a loss of rate in activity. This loss rate is less than five percent in the duration of the expiry date under a necessary storage condition. To have a lesser impact on the performance, the laboratory state and operating procedures, such as room temperature must be controlled.

Tumor marker for lung cancer. This is a sandwich type of kit with quantitative measurement of the tumor, other biological serum and in the plasma. This composed of perfect specificity that detects an antigen. It has a lower limit of detection that was contain the lowest concentration of protein that could be recognized from zero.

Early prostate cancer antigen 2 or the EPCA2. The components of this kit are reagents, pre coated, that is ready to use with ninety six well strip plate, plate sealer that is for 96 wells, standard or freeze dried, standard diluent, detection reagent, assay diluent A, assay diluent B, stop solution and wash buffer. The kits that came from different batches are different in sensitivity, detection range and color developing time.

A signal transduction assay reaction is a reliable method for detecting an amount of targets using the Phospho specific antibodies. It is commonly a solid phase sandwich enzyme that is providing a system for a target detection in a cell extract. The entire process of assay is not more than five hours.

Another is the ovarian cancer marker. The kit applies a competitive enzyme immunoassay technique that used a monoclonal antibody. The sample and the buffer are developed together within one hour. Once the incubation procedure was done, the wells are drained and washed for about five repetitions.

The DNA damage EIA is an aggressive colorimetric enzyme immunoassay that may result after 2.5 hours. The samples are already validated and convenient in the 96 wells plate scheme. The tests of DNA are measured through the serum, saliva and urine of a person. The types of samples are plasma, cell lysates and the DNA extracts.

These are just a few of the type of kits that is available for both human and animal detection for tumors. Since it has different kinds and specifications, it is still the decision of physician on how it would be treated.




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